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KMID : 0613820180280101220
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Journal of Life Science
2018 Volume.28 No. 10 p.1220 ~ p.1232
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Histological Comparative Study of Rabbit Maxillary Sinus Augmentation with Bio-Oss and β-TCP
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Moon Yong-Suk
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Abstract
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The purpose of this animal study was to evaluate, by histological analysis, bone regeneration in rabbit maxillary sinuses with an anorganic bovine graft (Bio-Oss) and a β-tricalcium phosphate (β-TCP) grafting. Bilateral sinus augmentation procedures were performed in 12 adult male rabbits. Rectangular replaceable bony windows were made with a piezoelectric thin saw insert. In the Bio-Oss group, Bio-Oss was grafted and in the β-TCP group, β-TCP was grafted and covered by replaceable bony windows. The animals were sacrificed at 2, 4, and 8 weeks after the surgical procedure. The augmented sinuses were evaluated by histomorphometric analysis using hematoxylin-eosin, Masson trichrome, and tartrate-resistant acid phosphatase stains and also by immunohistochemical analysis of proliferating cell nuclear antigen (PCNA), type I collagen, and osteocalcin content. Histologically, new bone formation was found on the surface of Bio-Oss and β-TCP particles from 2 weeks and continued to 8 weeks. Significant higher new bone formation was revealed in the β-TCP group than in the Bio-Oss group at 8 weeks. The amount of graft materials was significantly decreased in the β-TCP group and the number of osteoclasts was significantly increased in the β-TCP group from 4 to 8 weeks. Immunoreactivity to PCNA was reduced at 8 weeks. The expression of type I collagen was significantly increased in the β- TCP group at 2 weeks, but was significantly increased in the Bio-Oss group at 8 weeks. Immunoreactivity to osteocalcin was increased from 2 to 8 weeks. These histological results can help in the selection of graft materials for implants. Both Bio-Oss and β-TCP are proven graft materials, however, these results indicate that β-TCP showed better bone regeneration results in rabbit maxillary sinus augmentation.
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KEYWORD
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Bio-Oss, β-TCP, immunohistochemistry, maxillary sinus augmentation, new bone formation
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